FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_jo2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_jo2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14648744
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG40050.026.5224065
CGGGGGG12100.025.3670165
TCGGGGG11250.024.8312574
AGGGGGG21700.019.7072815
TTGGGGG40700.018.7268224
GCCGTCT65900.013.77686356
TATGCCG69550.013.64978953
TGCCGTC68050.013.49386955
GTATGCC69550.013.3516152
GTCACGC73100.013.02545336
GAGCGGT31300.013.00096810
TATCTCG71900.012.76708746
TAGGGGG13900.012.6538184
TCACGCC75950.012.40045137
ATGCCGT77500.012.33867454
AGTCACG80550.011.863811535
CTCGTAT80150.011.79880549
CACGCCA80900.011.68459138
AGAGCGG35550.011.4466649
CTGCTTG82000.011.40330564
Produced by FastQC (version 0.11.2)