FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_jo1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_jo1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19103298
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG51800.029.162295
CGGGGGG16450.026.4169065
TCGGGGG15050.024.9785544
AGGGGGG27450.019.6001575
TTGGGGG54400.019.4633124
TAGGGGG19300.010.9005854
TTTGGGG78500.010.5007233
ATGGGGG35150.010.106234
GAGCGGT35850.010.10082810
AGAGCGG40850.09.2864469
TTCGGGG26200.09.2148383
TGCCGAG34450.09.21198427
ATCGGGG20000.08.7949053
CCCTACG26700.08.01149440
CCCCGGA24600.07.9935941
AAGGGGG38350.07.9139744
CGGCCGG18900.07.665207426
GCGGTTC51200.07.611632312
GCCGAGA35950.07.3890128
AAGAGCG56600.07.2506538
Produced by FastQC (version 0.11.2)