FastQCFastQC Report
Thu 27 Jul 2017
HYFCVBGX2_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFCVBGX2_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410516932
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGAG11816550.068.914568
GTGTCGG146304950.068.8291856
TGTCGGT146304150.068.807647
TCGGTCT146374050.068.786929
CTCGAGG10206450.068.774439
GTCGGTC146391000.068.768358
CGGTCTC146449000.068.7408810
CCGGGTG1113700.068.699982
CTCGTAG146425750.068.6565314
GGTCTCG146588850.068.65575411
ACGAGGT132021500.068.6554910
GGTCTCT141733850.068.651314
GTCTCGT146933850.068.6048912
AGGTCTC142031550.068.58961513
GAGGTCT142426800.068.5447512
CACGAGG132200600.068.544139
CCACGAG132699250.068.533678
CGCCGGT2682050.068.495321
TCCACGA133011100.068.4183657
ACATGTG1703650.068.417252
Produced by FastQC (version 0.11.2)