FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10161496
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG195676919.256701965931No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG458350.45106547303664735No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG171520.16879404371167395No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC129270.12721552023442217Illumina Single End PCR Primer 1 (100% over 50bp)
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122440.12049406898354337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG216900.065.3381818
CACACGT188600.064.4263219
AGAGCAC239900.063.96550815
AGCACAC215100.063.63775617
ACACGTC134850.063.30023220
CACGTCG33950.062.72844321
TAACTGA145800.061.82682
GCACGCG28400.061.543618
ACTGAAG145500.060.6663134
CACGTCT89550.060.27507821
CGCGTCT26250.060.04597521
CACGCGT22300.059.5298919
GAGCACA241650.058.74794416
CGCGCGT31700.058.5621919
ACACGGC20800.058.2657720
CTGAAGA155200.058.1060185
GATCATC79300.057.9477210
CACACGG25550.057.1670619
AAGAGCA274900.056.1114414
CGTCGGA20050.055.6259823
Produced by FastQC (version 0.11.5)