FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n02_DS1285-05-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n02_DS1285-05-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21580994
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2762741.2801727297639767No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC979490.4538669534869432Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG137350.055.75112518
CACACGT122250.048.95321319
AGAGCAC226400.046.90089815
GAGCACA219750.046.13798516
AGCACAC189400.045.84413517
AAGAGCA329850.034.37728514
ACACGTC70300.034.05114420
GTGACTG80250.030.139491
ACACGTT77500.029.8942220
CGCCGTA333650.028.07994547
GACTGGA95250.027.691633
CCGTATC328000.027.35805349
TCGCCGT338650.027.25185446
GGTCGCC367950.027.0221244
CAGATCG168150.027.0196826
TGACTGG92750.026.8633142
GCCGTAT354650.026.78242548
GTCGCCG365800.026.49209245
CACACGA39150.026.10267619
CGTATCA353150.025.67729450
Produced by FastQC (version 0.11.5)