FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n02_DS1285-04-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n02_DS1285-04-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21163123
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1970880.9312803219071212No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC777790.36752137196386375Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG75900.045.41826218
CACACGT73400.037.66752619
CGCCGTA239950.031.89819747
AGAGCAC153650.031.61543315
TCGCCGT242750.031.08326546
GGTCGCC268750.030.87586644
GAGCACA149350.030.72094516
GTCGCCG272850.030.6813445
GCCGTAT265650.029.93205848
AGCACAC129550.029.6890517
CCGTATC251750.029.52725849
GTGGTCG284800.028.86550142
CTCGGTG264000.028.36898838
TCTCGGT272400.027.37860937
ATCTCGG280550.027.3566836
CGTATCA278600.027.33479750
GATCTCG279000.027.01938635
TGGTCGC300200.026.5220343
TCGGTGG314650.026.14929239
GGTGGTC313500.026.11131541
Produced by FastQC (version 0.11.5)