FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n02_DS1285-01-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n02_DS1285-01-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12888196
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1265520.9819217522762689No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC785550.6095112147580624Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG48550.044.4766718
CACACGT43350.037.9441719
CGCCGTA238900.034.2066447
GGTCGCC269100.033.6322744
AGAGCAC96650.033.5314415
TCGCCGT241250.033.32230446
GTCGCCG270900.033.31836745
GAGCACA93550.032.99624316
CCGTATC237700.032.75974349
GCCGTAT257000.032.5192848
GTGGTCG279300.032.1032942
CTCGGTG263400.031.595938
TGGTCGC278250.031.11758643
CGTATCA255750.031.04990450
AGCACAC80350.030.88149817
TCTCGGT268800.030.59636737
ATCTCGG277150.030.44495236
GATCTCG273400.030.37611235
GGTGGTC301750.029.74950641
TCGGTGG306050.029.57143839
Produced by FastQC (version 0.11.5)