FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n02_DS1285-00-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n02_DS1285-00-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16257236
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1534640.9439735020147336No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC955620.5878120979482613Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG59100.045.59743518
CACACGT56150.036.21291719
AGAGCAC119300.034.08862715
CGCCGTA297400.032.97374747
GAGCACA117900.032.7121416
CCGTATC298050.031.96255549
GCCGTAT316100.031.89783748
TCGCCGT304750.031.87979946
AGCACAC97400.031.87164317
GGTCGCC320650.031.71778344
GTCGCCG323050.031.46057745
CGTATCA314700.030.44952450
GTGGTCG337350.030.12689442
CTCGGTG304650.029.91416738
TCTCGGT310850.029.17106837
ATCTCGG314100.029.10312836
TGGTCGC346200.029.09391643
GATCTCG309600.028.49747535
GGTGGTC360800.028.01361341
TCGGTGG355000.027.86984639
Produced by FastQC (version 0.11.5)