FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n02_DS1281-05-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n02_DS1281-05-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17357278
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1747211.0066152077531971No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC673860.388229076010651Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG82900.051.63065318
AGCACAC110250.043.86987717
GAGCACA129400.043.40904616
CACACGT80900.042.91398219
AGAGCAC137050.042.79920215
ACACGTC48600.030.53270520
CGCCGTA211400.030.46148347
GGTCGCC228700.029.50370644
TCGCCGT217150.029.39684146
GCCGTAT228700.029.01418348
GTCGCCG232900.028.9265745
CCGTATC214400.028.84363449
GTGGTCG244850.027.35756942
CTCGGTG221600.026.76913838
AAGAGCA236950.026.45343614
TCTCGGT227100.025.82803337
ATCTCGG230900.025.69095436
CGTATCA244950.025.47487650
GGTGGTC265750.025.24545941
GATCTCG230600.025.13241635
Produced by FastQC (version 0.11.5)