FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n02_DS1281-04-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n02_DS1281-04-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18934153
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1734080.9158476748339364No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC445360.2352151691179426Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG68350.046.13417418
AGCACAC94300.038.63482717
GAGCACA113900.038.1936216
AGAGCAC120600.038.10344315
CACACGT69150.036.33864619
GGTCGCC160400.030.26268444
CGCCGTA144500.029.9597647
GTCGCCG165050.029.32526845
TCGCCGT151700.028.69922346
GCCGTAT164000.027.5072248
GTGGTCG177500.027.4457542
CTCGGTG164550.027.24484438
CCGTATC157600.026.78111849
ATCTCGG174350.025.95424836
TCTCGGT172000.025.75941537
GATCTCG177450.025.1261835
GGTGGTC198000.024.48035241
TCGGTGG203300.024.0832239
TGGTCGC206000.022.8841443
CGTATCA188800.022.83738350
Produced by FastQC (version 0.11.5)