FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n02_DS1281-01-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n02_DS1281-01-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19302356
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1845550.9561268064893219No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1000620.5183926770390102Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG82050.047.60177218
GAGCACA138350.040.0191816
AGAGCAC146450.039.8613515
AGCACAC113350.039.39742317
CACACGT76700.038.87601519
AGATAGA177500.027.9220587
ATAGATC177900.027.8573279
GATAGAT179000.027.823228
CGCCGTA349000.027.45652247
ACACGTC47100.026.97262620
TCGCCGT358600.026.60430746
CCGTATC345700.026.50377749
GCCGTAT368050.026.45378348
GGTCGCC382250.026.30409844
GTCGCCG382700.026.11777145
GATCGAG47400.025.400118
GTGACTG62750.025.306851
GTGGTCG395200.025.2207742
AAGAGCA257700.024.90763714
CGTATCA377500.024.51218250
Produced by FastQC (version 0.11.5)