FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n02_DS1281-00-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n02_DS1281-00-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19701423
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2140001.086215955060708Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2046941.03898078834204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG103950.050.87172318
GAGCACA169000.045.10391616
AGCACAC131400.044.9587917
AGAGCAC177100.044.8004815
CACACGT85800.043.1960519
GATAGAT251950.038.229948
AGATAGA256050.037.633177
ATAGATC263050.036.7226269
GATCGAG61900.036.1875238
GTGACTG81100.033.6264761
AAGAGCA289250.030.32212314
CGCCGTA703200.030.24445547
ACACGTC51950.029.91097620
TGACTGG90200.029.8744542
CCGTATC683550.029.6906249
GACTGGA92850.029.688463
TCGCCGT714150.029.5356946
CAGATAG110650.029.5146986
GCCGTAT735150.029.39178848
GGTCGCC764450.028.97936644
Produced by FastQC (version 0.11.5)