FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10161496
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1174041.1553810580646786No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA1134411.1163808950965488TruSeq Adapter, Index 14 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG35950.033.1932331
CACAGAT299300.031.77633932
TGACTGG38450.031.6491952
TCACAGA296550.031.6279331
ACAGATC305500.031.12144933
GTCACAG295050.031.05045930
CTTAGGC38300.028.04246168
ACTTAGG38650.027.97055867
CAGATCG361850.026.24197234
TTAGGCA41800.025.18697769
CCGATGT38650.025.15496668
AGTCACT104950.025.12063863
ACCGATG40550.024.14978667
AGTCACC105350.023.69235263
ACACTTG95800.023.44846367
CAGATCA61150.023.41503369
ACTGGAG52600.022.935994
CACTTGA97950.022.79009668
GTCACCG44100.022.45599264
CACACTT100600.022.37496466
Produced by FastQC (version 0.11.5)