FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n01_DS1285-05-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n01_DS1285-05-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21580994
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1179710.5466430322903569TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA221560.10266440924824871TruSeq Adapter, Index 14 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC70250.027.60109516
CGACGCT69150.027.58463317
CTACACG70250.027.45163212
ACACGAC76900.025.6238814
CCTACAC75400.025.57661811
TGCCGTC366400.025.45684649
GCCGTCT335000.025.32507150
TATGCCG386400.025.24426347
ATGCCGT385150.024.9172748
CTGCTTG316850.024.09166558
GTATGCC411800.024.01865646
CCGTCTT356850.023.95095451
CCCTACA82650.023.54478810
CTCGTAT394150.023.32713543
TCCCTAC83900.023.2357189
TGCTTGA336950.023.20505359
GATCTCG385250.023.20283940
CGTATGC426100.023.17151645
GCTTGAA366400.022.39066760
CGATCTC401700.022.26137439
Produced by FastQC (version 0.11.5)