FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n01_DS1285-04-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n01_DS1285-04-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21163123
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT59550.024.97874617
CTACACG61200.024.70562212
ACGACGC62350.024.1938116
TATGCCG101550.023.78113747
TATCGCG104150.023.65793440
CCGATGT425200.023.5827734
GCGGGGT47150.023.45680451
TATCGGG132800.023.19252440
CCTACAC66400.023.08710511
CGATGTA435700.022.89395135
ACCGATG443300.022.74620633
ACACGAC66950.022.63605514
CACCGAT454650.022.3092332
TGCCGGC54050.022.27543349
ATCGGGG90350.022.23551441
GTCACCG457300.022.19526130
CCCTACA69850.022.09711810
TCACCGA461700.022.00648131
GCCGGCG32300.021.99667250
ACGTCTG525150.021.9468316
Produced by FastQC (version 0.11.5)