FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n01_DS1285-01-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n01_DS1285-01-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12888196
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG245390.19039902869261144TruSeq Adapter, Index 3 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT42600.029.9879417
ACGACGC43350.029.63059416
CTACACG43900.028.94046212
CATCGCG79750.028.30690640
CCTACAC46500.027.6233611
AGGCATC274100.027.40211537
ACACGAC47500.027.33654614
TATGCCG101100.027.10653347
TGCCGTC87900.026.59812249
GGCATCG145700.026.32778738
TAGGCAT393600.025.94738436
CTTAGGC420200.025.81243134
CATCTCG115000.025.80841640
GTATGCC112950.025.75005546
GCCGTCT66900.025.73963550
GCGGGGT44600.025.66116151
ACTTAGG425400.025.58740833
GCGTGAA55400.025.58636760
CCCTACA49950.025.57530210
TTAGGCA426450.025.23715435
Produced by FastQC (version 0.11.5)