FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n01_DS1285-00-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n01_DS1285-00-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16257236
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1094840.6734478111777427TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT255450.030.14258850
TGCCGTC292300.030.01861249
TATGCCG315400.029.55116547
ATGCCGT304450.029.36094348
GTATGCC330150.028.54895246
CCGTCTT276550.028.38699251
CTCGTAT318200.028.20219843
CTGCTTG241250.028.144858
CGACGCT55750.027.87416517
CGTATGC340600.027.58055545
ACGACGC57600.027.22195216
TGCTTGA258800.027.07469659
ATCTCGT339250.027.06089841
AATCTCG349750.026.7188240
CTACACG59900.026.52728512
GCTTGAA284900.026.47394460
TCGTATG353200.026.46782944
TCTCGTA334900.026.46145242
ACACGAC62850.025.7276714
CCTACAC61800.025.48518811
Produced by FastQC (version 0.11.5)