FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n01_DS1281-05-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n01_DS1281-05-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17357278
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG101900.023.21861647
GTCACAC335050.022.62625530
CACACAG336200.022.51762832
GTATGCC112700.021.89419746
ACACAGT346850.021.76568233
GCGTGAA57150.021.74082260
CACAGTG350400.021.61508834
TGCCGTC85600.021.42502649
TGCCGGC46900.021.19382349
ATGCCGG53500.021.06521648
GAACTCC389350.021.03485522
CAGTCAC362350.020.96985228
ACGTCTG400900.020.94392216
GCACACG402250.020.94324112
ACACGTC401600.020.93356114
CACACGT402850.020.8599213
AGTCACA366500.020.81835229
CACGTCT406250.020.65949215
CCAGTCA370950.020.60635227
TCCAGTC377550.020.47788426
Produced by FastQC (version 0.11.5)