FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n01_DS1281-04-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n01_DS1281-04-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18934153
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG590770.31201290070910487TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG172700.025.94066447
GCCGTCT141950.025.07546650
ATGCCGT168000.025.04140348
TGCCGTC165350.024.61721849
GTATGCC185900.024.4564446
GTCACGC249800.023.87484730
TCACGCC252050.023.63395131
CGTATGC193450.023.30293345
CACGCCA257250.023.25145532
TATCTCG212150.023.04712940
AGTCACG261250.022.9490429
CTCGTAT200650.022.11788443
CCGTCTT167300.022.02905551
ACGCCAA286550.021.0205433
TCGTATG208900.020.90930644
GCACACG319900.020.89693512
ATCTCGT231400.020.87272641
ACACGTC320850.020.83506214
CAGTCAC290650.020.7962728
GAACTCC314350.020.76485322
Produced by FastQC (version 0.11.5)