FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n01_DS1281-01-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n01_DS1281-01-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19302356
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGCG107350.022.72447440
GCGGGGT71950.021.8413151
CATCGGG149200.021.2531140
ATCGGGG113600.020.98127641
ATCGCGT69900.020.17859841
CAGATCA533850.020.1730435
AGTCACC555200.019.78183629
TGCGGGG40900.019.76750450
ACGTCTG642550.019.59825316
GAACTCC624550.019.52423722
ACACGTC646300.019.47912214
GCACACG646800.019.44783212
CACGTCT648350.019.41753415
CAGTCAC574600.019.31495728
CACACGT651050.019.31012713
GCGTGAA61650.019.24557560
GATCATC456500.019.20569637
AGATCAT532000.019.16425736
CGTCTGA658250.019.1201817
CCAGTCA587700.019.1166827
Produced by FastQC (version 0.11.5)