FastQCFastQC Report
Tue 17 Dec 2019
HYCYVAFXY_n01_DS1281-00-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCYVAFXY_n01_DS1281-00-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19701423
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTG356730.1810681390882273TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTT282220.14324853590524908TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTG249240.12650862833613594TruSeq Adapter, Index 8 (97% over 42bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG170250.026.9307140
TGCGGGG89450.026.37200550
TCGGGGA69650.026.1806442
GCGGGGT165000.026.09064551
ATCGCGG106200.025.77186841
CGGGGAT68300.025.10955443
TTGCGGG100300.025.08947849
ATCGGGG433000.024.85540641
TCGGGGT210000.024.76641542
CGGGGTT196800.024.70249443
AATCGGG527750.024.68378440
TGAATCG748900.024.40020638
GAATCGG556850.023.94067639
CGGGGTG201800.023.83028452
GTCACAC1046150.023.78697830
GCCGGCG42250.023.77490650
GCGGCGT66200.023.68556251
CGGCGTG78700.023.65922552
ATCGCGT78200.023.49720441
CCGGCGT50100.023.47281651
Produced by FastQC (version 0.11.5)