FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10938344
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7754397.089180958287653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA36150.023.13848553
CCGTATC34900.022.96420355
TCGCCGT37000.022.12911452
GCCGTAT40200.021.67796954
CTCGGTG37000.021.561844
CGTATCA38950.021.29522556
TCTCGGT37300.020.9192443
GATCTCG39350.019.9189841
GTATCAT43000.018.3940557
AGATCTC44150.018.1500840
TCGGTGG53200.017.75865245
CCGGATC4600.017.49892455
AAGATCG39300.016.6641356
CGGGAAG19400.016.6435491
CGAGATC9900.016.6296835
TAGATCT48900.016.53083439
CGGATCA5250.015.3323956
CTAGATC10550.015.2730815
TCGCCGG6500.015.07282352
TCATTAA63500.014.82328160
Produced by FastQC (version 0.11.5)