FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n02_ncm7-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n02_ncm7-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14796491
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG330720.22351245305390313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA225300.021.38667553
CCGTATC212950.021.27941555
CGTATCA218350.020.81725756
TCGCCGT234150.020.56394852
CGGCAAG37400.020.2681241
GGTCGCC250900.020.20999150
GTCGCCG245450.020.07398651
GCCGTAT245800.020.00154354
TGGTCGC254600.019.71004749
CTCGGTG222450.019.4116344
TCTCGGT225900.018.9449743
ATCTCGG227850.018.72140342
GTGGTCG270450.018.64536948
CGCGAAG20750.018.6039831
GATCTCG227800.018.41909441
GTATCAT242650.017.83832257
GGTGGTC283500.017.61439947
CGCCAAA60900.017.2300011
AGATCTC248500.017.1102840
TCGGTGG283550.016.9813645
Produced by FastQC (version 0.11.5)