FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n02_ncm7-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n02_ncm7-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13702282
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG342980.25030867121257616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG19550.019.9257261
CGCCGTA217550.019.9125553
CCGTATC203300.019.8967755
TCGCCGT221450.019.45154252
CGTATCA211100.019.34400456
GTCGCCG231200.019.34344151
GCCGTAT233450.019.09593254
GGTCGCC241400.018.9466450
CGGCAAG44700.018.8426361
TGGTCGC247000.018.34700249
CTCGGTG221150.017.91172844
TCTCGGT226550.017.46959743
GTATCAT225400.017.3093857
GTGGTCG262750.017.27380248
ATCTCGG228850.017.23273342
GATCTCG227400.016.97410441
CGGGAAG83500.016.6435781
CGCCAAG31100.016.5880321
GGTGGTC279450.016.2165347
TCATTAA260200.015.93548560
Produced by FastQC (version 0.11.5)