FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n02_ncm7-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n02_ncm7-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14324431
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG412590.28803238327581737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA219350.021.40784553
CCGTATC204200.021.26498255
TCGCCGT224800.020.95197952
CGTATCA210050.020.70605756
GTCGCCG234450.020.67247851
GCCGTAT230950.020.57485854
GGTCGCC245200.020.30869350
TGGTCGC248100.019.80324649
CTCGGTG218700.019.21599844
CGCGAAG20550.018.9562911
GTGGTCG263800.018.82363948
TCTCGGT225450.018.51656543
GTATCAT229050.018.3774157
ATCTCGG228150.018.28203242
GATCTCG228800.017.9251641
CGGGAAG82750.017.5579811
GGTGGTC281900.017.5529647
CGGCAAA87100.017.1243081
CGGCAAG43050.017.1193941
CGGCCAG11750.017.0246931
Produced by FastQC (version 0.11.5)