FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n02_ncm7-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n02_ncm7-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13420579
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG331710.24716519309636342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA143250.022.10661153
GGTCGCC159150.021.92220550
GTCGCCG158150.021.88364251
TCGCCGT145200.021.66553552
CCGTATC143100.021.44494455
TGGTCGC161200.020.94874649
CGTATCA148500.020.877256
GCCGTAT158650.020.86488754
CTCGGTG158350.020.04350544
GTGGTCG175000.019.71649748
CGCGAAG18650.019.5702341
ATCTCGG163200.019.53361542
TCTCGGT162950.019.34890743
GATCTCG167450.019.01779241
GGTGGTC190350.018.05327447
GTATCAT174050.017.63135357
CGGCAAG41400.017.462581
TCGGTGG202000.017.20219645
CGCGGAG12700.017.1328451
CGGGAAG75550.017.094441
Produced by FastQC (version 0.11.5)