FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n02_ncm7-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n02_ncm7-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13488761
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG312370.23157797814046818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA139200.021.36624153
CCGTATC135400.020.72530455
TCGCCGT143850.020.67603152
GTCGCCG147350.020.66146351
CGCGAAG17950.020.530751
GGTCGCC152450.020.4982950
GCCGTAT152450.020.06007254
TGGTCGC158000.019.64536549
CGTATCA144100.019.57100156
CGGCAAG40400.019.460171
CTCGGTG144700.018.76574544
GTGGTCG169350.018.53507648
TCTCGGT148150.018.35249943
ATCTCGG149900.018.16138542
GATCTCG151350.017.503341
CGGGAAG79300.017.1284391
CGGCAAA80700.017.0922411
CGGGCAG13350.016.8259161
GGTGGTC187800.016.6586147
GTATCAT167500.016.39822657
Produced by FastQC (version 0.11.5)