FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n02_ncm7-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n02_ncm7-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9756611
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG292060.2993457461817428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG12050.023.8807031
CGAGATC26900.023.6988245
CGGCAAG23150.021.374131
CGTATCA241550.020.58437556
CCGTATC242100.020.49425555
CGCCGTA263100.020.22838853
CGCGCAG4050.019.9293521
TCGCCGT269300.019.69781152
GCCGTAT282450.019.32573154
GTCGCCG283950.019.29945851
GGTCGCC296000.018.86845850
CATTAAA268800.018.65342761
TGTCGGC17600.018.49330530
GTATCAT255650.018.4088757
TGGTCGC301250.018.28407149
CGGGCAG8350.018.0718361
TCATTAA276700.017.81746160
CGCGGAG9100.017.7393131
CTCGGTG266650.017.67633644
CGAGCAG18250.017.4984231
Produced by FastQC (version 0.11.5)