FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n02_ncm7-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n02_ncm7-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7733952
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250250.32357325207086884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC155550.022.92222455
CGCCGTA158650.022.87161653
TCGCCGT161550.022.5261752
CGTATCA159400.022.52224356
CGAGATC20500.022.2146685
GCGAGCG8850.022.1471711
GTCGCCG179700.021.8492651
GGTCGCC182850.021.64523950
GCCGTAT176850.021.6060754
TGGTCGC180900.021.0272749
CGGCAAG16700.020.5945051
CGCGAAG9250.020.4877261
AAAGGGG74800.020.44308770
CTCGGTG177150.020.14770744
CAGATCG37600.020.0309245
ATCTCGG182050.019.97048842
TCTCGGT179250.019.83358443
GATCTCG181900.019.6801941
GTGGTCG195600.019.67957948
AGATCTC187100.019.09631240
Produced by FastQC (version 0.11.5)