FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n02_ncm7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n02_ncm7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8437856
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258940.30687890383528704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAG3350.027.2373581
CGCCGTA121200.023.09596653
CCGTATC118550.022.6971355
TCGCCGT124600.022.60682152
CGTATCA120750.022.42822356
GTCGCCG135050.022.28443351
GGTCGCC137650.021.93991350
CGAGATC17800.021.646655
TGGTCGC138200.021.4727749
GCCGTAT135250.021.31749354
GCGAGCG12900.021.16413711
ATCTCGG134250.020.33069642
CTCGGTG132050.020.29830444
GTGGTCG148500.020.24227148
GATCTCG135000.019.9867241
TCTCGGT135200.019.92890443
CGCGAAG8750.019.2517241
AGATCTC141350.019.08883740
CGGCAAG16950.019.0482271
GGTGGTC160900.018.70435347
Produced by FastQC (version 0.11.5)