FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n02_ncm7-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n02_ncm7-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7602270
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG195210.2567785674541946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCC2650.027.72774166
CGCGAAG9400.023.899291
CGGGCAG6550.023.0441151
CGCGGAG5900.022.013171
CGAGATC17100.021.71735
CGCCGTA142250.021.5231353
CCGTATC135450.021.5186855
CGTATCA137800.021.22802456
TCGCCGT145050.021.05982452
GCGAGCG11200.020.93675611
CGGCAAG18950.020.746351
GCCGTAT156250.020.55759454
GTCGCCG154500.020.4979251
GGTCGCC160950.020.41583450
TGGTCGC165200.019.6574749
CGGCCTA3950.019.5505281
GGCGAGC12250.019.42928910
CTCGGTG141600.019.39885544
ATCTCGG144500.019.22735242
CGCGAAA12050.019.2260381
Produced by FastQC (version 0.11.5)