FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n02_ncm7-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n02_ncm7-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15734357
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG415660.26417348989857037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAG40000.020.3572431
CGCGAAG21300.019.114781
CGGGAAG90900.018.495351
CGCGAAA29000.018.1544971
CGCGGAG10350.017.6341251
TGTCGGC31600.017.49881630
CGGCGGC32900.017.33831433
CGCCAAG42800.016.7292821
GTCGGCG34650.015.95805931
CGCCAAA63950.015.8616471
CGGCAAA105100.015.7292911
CGGGAAA65050.015.6473791
TCGGCGG36350.015.499927532
CGGGCTG8850.015.4672211
CGGGCAG16900.014.74560451
CGCCGTA271900.014.67113853
CGAGCAG28600.014.6040011
CCGTATC257650.014.51825355
TAGTCCG48250.014.50803611
CGGCAAT43800.014.5042541
Produced by FastQC (version 0.11.5)