FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n02_ncm7-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n02_ncm7-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11139659
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG287370.25797019459931403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG16600.020.5039561
CGGCAAG31300.019.8427981
CGCCGTA92200.018.10297653
CCGTATC98150.017.54031255
CGCGAAA21350.017.4213561
GGTCGCC116750.017.3844550
CGGGAAG62600.017.2083031
GTCGCCG117200.017.1684151
TCGCCGT97000.017.06324852
CGCCAAA48350.016.8370321
TGGTCGC111550.016.68913549
TGTCGGC21550.016.5647830
CGTATCA108150.016.46841656
GCCGTAT113850.016.41242454
CGGCGGC21700.016.28863533
CGCGAAT13600.015.9965671
CTCGGTG121700.015.84320644
CGAGCAG20450.015.7858691
ATCTCGG120600.015.75537442
TCTCGGT122500.015.482717543
Produced by FastQC (version 0.11.5)