FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n02_ncm7-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n02_ncm7-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13794196
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG373990.27112127448384815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC276100.020.69623855
CGCCGTA286400.020.56289753
CGTATCA280550.020.52988256
CGGCAAG37100.020.1497571
TCGCCGT294150.020.03331252
CGAGATC42200.019.9208165
GCCGTAT310550.019.6848954
GTCGCCG317250.019.51281451
GGTCGCC325850.019.25577450
TGGTCGC327900.018.80454849
GTATCAT293150.018.6685557
CTCGGTG299500.018.36700844
CGCGAAA25800.018.2284281
TCTCGGT303700.018.00936743
CGGGAAG75850.017.9531351
GTGGTCG347300.017.87446648
ATCTCGG310450.017.75292842
GATCTCG310350.017.4775941
CATTAAA340600.017.35146561
TCATTAA339550.017.29176760
Produced by FastQC (version 0.11.5)