FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10938344
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT54900.020.6026731
GAGATCG47100.019.991896
AAGATCG74500.017.2907586
ACACTCT54350.015.5278321
CACACGT170000.015.49031619
GCTTGAA55650.015.28556566
ACGTCTG165850.015.27519322
CGTCTGA165800.015.25855623
ACACGTC171200.015.15590820
GCACACG173350.015.15160418
CACGTCT172550.014.82422421
AGAGCAC186050.014.7405915
GATCGGA195200.014.69772158
AGCACAC184300.014.68876517
AGATCGG197300.014.6645447
TCGGAAG200300.014.27330710
CGGAAGA201750.014.18845711
CTGAACT173850.014.15031326
TGAACTC174850.014.10941427
GAACTCC167250.013.97456228
Produced by FastQC (version 0.11.5)