FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n01_ncm7-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n01_ncm7-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14796491
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG216400.024.32370664
ACACTCT77000.024.0139031
GCTTGAA229500.023.56036666
TGCTTGA232750.022.87073765
GCCGTCT258950.021.51725456
TGCCGTC286150.019.86393255
CCGTCTT282900.019.78229557
ATGCCGT297300.019.18958754
TATGCCG301850.018.9235253
GTATGCC312700.018.34538852
TCTGCTT292100.018.30764263
CGTATGC317250.018.00432251
GAGATCG108400.017.9576936
CTTGAAA308400.017.87320367
CACTCTT150200.017.6267171
CTTCTGC315650.017.29637361
TCGTATG331850.017.15976350
CTCGTAT331400.016.68685749
GATCTCG324550.016.57566846
TTCTGCT324600.016.53925762
Produced by FastQC (version 0.11.5)