FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n01_ncm7-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n01_ncm7-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13702282
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT73650.027.1955181
GCGTGAA36500.017.35508566
TGCCGGC38250.016.28775655
GCCGGCG24550.016.109856
CGGGGTG45900.015.936583558
GCGGGGT41300.015.84722357
CCGGCGT32750.015.81655957
CACTCTT170750.015.688261
GAGATCG116750.015.6525886
GGGTGTA31950.015.44504766
CTGCGTG29050.014.93888364
AATCGCG93150.014.91723346
AGGGGGG16150.014.7438341
TGCGTGA29050.014.57745865
TGCCGGG26300.014.10661555
CCGGGGT23700.014.02932857
GTGGGGG47150.013.88041662
TGGGGGT37350.013.86799663
GGGTGGA25400.013.7786766
GTCACAC381000.013.69799736
Produced by FastQC (version 0.11.5)