FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n01_ncm7-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n01_ncm7-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14324431
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT75750.025.1466261
GCGGGGT40500.017.80171457
CGGGGTG47800.017.79213158
TGCCGGC41750.016.93430155
GCGTGAA40050.016.77790566
TGGCGGG36800.016.73929855
GGGTGGG42850.016.17161660
GCCGGCG25500.015.92100556
TATGCCG73100.015.84825553
GAGATCG108650.015.8204256
AGGGGGG17850.015.6933341
CATCTCG109800.015.46078346
CCGGCGT29550.015.2785457
GTGGGGG46750.015.27176162
GGGTGGA28000.015.248980566
GGCGGGG36050.015.24217956
ATGCCGG38750.014.99369854
CAGATCA367050.014.90442541
GTATGCC84850.014.68488552
AGTCACC385450.014.62064735
Produced by FastQC (version 0.11.5)