FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n01_ncm7-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n01_ncm7-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13420579
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT154750.021.71182656
CTGCTTG144550.020.65224564
GCTTGAA156800.020.44455566
TGCCGTC182000.020.0384955
TATGCCG190700.019.63820553
CCGTCTT176900.019.36922557
ATGCCGT190400.019.1544454
ACACTCT58700.018.911931
TGCTTGA159450.018.87601365
GTATGCC204850.018.72606552
CGTATGC213050.017.93900951
AGGGGGG17750.017.5592671
TCGTATG223600.016.98347950
CTCGTAT227200.016.94576549
TATCTCG241900.016.85660446
GAGATCG86750.016.8298136
GTCACGC254700.016.6836436
ATCTCGT246050.016.58639347
TCTCGTA236100.016.55899448
TCACGCC258250.016.44080737
Produced by FastQC (version 0.11.5)