FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n01_ncm7-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n01_ncm7-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13488761
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT56950.018.5090431
GCCGTCT53550.017.71215256
TATGCCG86250.017.49024253
GCGTGAA40050.017.47679166
GTATGCC99550.016.0676652
TGCCGTC78800.015.67916555
TGCGTGA32400.015.55434265
CACAGTG234400.015.34988440
GTCACAC240350.015.00023736
TGCCGGC35000.015.0002255
CGTATGC103650.014.99259651
GAGATCG79850.014.9066446
AGGGGGG18050.014.7450931
ATGCCGT85350.014.43554
ACACAGT252200.014.36434639
CTGCGTG32250.014.32446564
GCCGGCG20100.014.27839456
CACACAG255300.014.2585838
AGTCACA256050.014.06675835
CAGTGAT250000.013.98615542
Produced by FastQC (version 0.11.5)