FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n01_ncm7-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n01_ncm7-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9756611
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT62200.036.8207241
GGGATTC34850.027.8191432
GCTTGAA186350.026.38667766
CTGCTTG180750.025.98449164
TGCTTGA189550.024.96277665
CACTCTT143750.023.8251651
GTTGGGA42600.023.74320629
TGTTGGG44000.022.6693428
GGCTATA39350.022.59251852
CTTGAAA230200.021.9076867
TGGGATT47500.021.2947831
GCGTGAA27700.021.22595866
TCTGCTT227300.021.03251663
GGATTCC48550.020.18707333
GCCGTCT258300.019.97232656
AGGCTAT44900.019.79959551
CCGTCTT267100.019.56327257
CTTCTGC254650.019.4195261
GCTATAA46600.019.00228353
TAAAGGC46250.018.99541348
Produced by FastQC (version 0.11.5)