FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n01_ncm7-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n01_ncm7-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7733952
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT37850.032.19661
GCGTGAA29500.024.79394766
GGGTGTA14450.023.0079166
CTGCGTG21050.022.44487264
CACTCTT85350.021.622391
GGCGTGA10950.021.09430765
CGTGAAA37500.020.99774667
GAGATCG76550.020.5350936
TGCCGGC28450.020.42237355
GCCGTCT53000.019.87650956
TGCGTGA23900.019.1826665
CTGCTTG48850.019.12855164
TATGCCG83700.018.90137153
GTCGGGG5000.018.89870362
GCCGGCG16450.018.72260356
TGCCGTC72400.018.70909555
GCCGGCT15900.018.48977756
CCGGCGT19100.018.3240657
GCGTGGG10700.018.316560
CGGGGTG25000.018.19945758
Produced by FastQC (version 0.11.5)