FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n01_ncm7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n01_ncm7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8437856
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT37600.031.8522571
GCGTGAA25750.028.40504866
GCCGGCG14750.025.15162556
TGCCGGC27250.023.7618755
TGCGTGA22700.023.5884765
CCGGCGT18500.023.4587457
CTGCGTG20600.022.42543664
ATGCCGG26750.022.11265254
CGTGAAA35250.021.84187967
GGCGTGA9150.021.0366165
TATGCCG61400.021.03455553
GAGATCG58650.021.0122996
GTGCGCG5400.020.73838611
GCCGTCT37050.020.12072256
GTGCGTG11500.019.47674264
CACTCTT82400.019.209371
TGCCGTC50300.018.9267655
GGGCGTG7700.018.63490964
GCGCGAT5300.018.48868613
GCCGGCT15750.018.22155856
Produced by FastQC (version 0.11.5)