FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n01_ncm7-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n01_ncm7-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7602270
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT39550.033.6482621
GCTTGAA122800.026.7326166
CTGCTTG118300.026.56631964
TGCTTGA124700.025.53963765
GCCGTCT161750.021.37812256
TCTGCTT151000.021.20708863
CTTGAAA158950.021.18124467
CACTCTT91150.020.8626121
CCGTCTT167950.020.6514557
TGCCGTC179650.020.04732755
GAGATCG60550.019.8331266
GTCTTCT170800.019.54790759
CTTCTGC173250.019.21061761
TATGCCG188250.019.16879853
ATGCCGT188500.019.16181654
CGCGGAC4950.019.09117146
CGTCTTC186350.018.53722458
TTCTGCT174650.018.07485862
GTATGCC200900.018.03137252
CGTATGC203950.017.77817751
Produced by FastQC (version 0.11.5)