FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n01_ncm7-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n01_ncm7-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15734357
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT96700.029.94821
CACTCTT196300.017.8747141
GCGTGAA47900.016.95074566
CTGCTTG133250.014.5245964
CTGCGTG39600.014.40583364
GCTTGAA152450.013.93469866
TGCGTGA43550.013.82244565
AGGGGGG17050.013.7607511
GGCGGTA26550.013.578542
GCCGTCT163000.013.46284856
AGTGGCG26500.013.20842839
GCGGTAG27700.012.76213843
TGCTTGA152800.012.666265
TATGCCG231600.012.64904553
GTGGCGG27750.012.61309440
TGCCGTC207300.012.39278555
CGTGAAA68700.012.277122567
ATGCCGT211150.012.03425154
GTATGCC251250.011.99410652
TGCCGGC41850.011.95949455
Produced by FastQC (version 0.11.5)