FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n01_ncm7-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n01_ncm7-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11139659
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT58650.026.6300051
CTGCTTG133500.019.45180364
CACTCTT121750.019.3000261
GCTTGAA143150.019.0693866
TGCTTGA147000.017.76072365
GCCGTCT173100.016.72096356
CCGTCTT190050.015.5978457
TGCCGTC203300.015.25353955
TATGCCG215800.014.66186753
ATGCCGT210100.014.64323654
AGGGGGG12350.014.1777531
GTATGCC227600.014.11701152
TCTGCTT192600.013.9736363
CTTGAAA203400.013.90252767
CGTATGC233050.013.72630951
GCGTGAA23650.013.46615366
GAGATCG84750.013.3433776
CTTCTGC207050.013.21809161
TCGTATG242600.013.08533850
CTCGTAT247700.012.8584849
Produced by FastQC (version 0.11.5)