FastQCFastQC Report
Tue 10 Dec 2019
HYCY3AFXY_n01_ncm7-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCY3AFXY_n01_ncm7-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13794196
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT80350.030.462361
GCGTGAA46550.021.42699266
CACTCTT165450.020.1061571
CTGCGTG34750.020.04183464
GGGTGTA35400.019.17937966
TGCCGGC51350.019.01632555
TGCGTGA37950.018.444165
CCGGCGT40400.018.19219257
GCCGGCG32700.017.76685556
GAGATCG135450.017.7303836
ATGCCGG44700.017.30429554
CGTGAAA62950.017.2902367
TGCCGGG32300.016.57874955
CGGGGTG54950.016.24125558
CCGGGGT28900.016.2275957
GCCGGGG24350.016.09790256
GCGTGGG22400.015.93638560
GTCGGGG9950.015.82815362
GTGCGTG22500.015.71006864
GGCGTGA18550.015.65933865
Produced by FastQC (version 0.11.5)