FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11377992
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7847696.897253926703412No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG124800.10968543482892236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC44450.027.56014855
CGCCGTA48800.026.03598653
CGTATCA49950.025.92706956
TCGCCGT49400.025.36549252
GCCGTAT53550.025.36070654
TCTCGGT49850.024.22224443
GATCTCG48950.023.73725941
CTCGGTG52600.023.3553144
GTATCAT54350.022.47549457
AGATCTC54200.022.27624940
CGAGATC16600.020.8761585
TAGATCT64150.019.74850339
TCATTAA74200.018.53757560
GATCGGA134600.018.1993628
AGATCGG138350.018.1620837
AAGATCG54200.018.0163696
ATCGGAA140600.017.7213139
TATCATT71000.017.69757358
AGCGTCG131800.017.3658517
TCGGAAG140900.017.23637410
Produced by FastQC (version 0.11.5)