FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n02_ncm6-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n02_ncm6-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11590711
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG179410.15478774339210077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAG32250.021.9572371
CCGTATC185800.020.57208655
CGTATCA192750.020.10270956
CGCCGTA198450.020.05470853
TCGCCGT200400.019.580152
GGTCGCC215900.019.24401750
GCCGTAT215350.019.19605854
GTCGCCG216900.019.05863251
TGGTCGC218250.018.7158149
TGTCGGC21900.018.6957930
CTCGGTG196300.018.3292344
CGCGAAA20800.018.201891
GTATCAT207500.017.8806657
TCTCGGT200950.017.80035443
CGGGAAG62950.017.764421
GTGGTCG231650.017.73894748
GATCTCG198250.017.67127641
ATCTCGG204800.017.67011842
CGCCAAA46050.017.5848391
GGTGGTC239150.017.21160947
Produced by FastQC (version 0.11.5)