FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n02_ncm6-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n02_ncm6-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13769959
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG219610.15948486121127883No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG151580.11008021156780495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC309600.026.38634755
CGCCGTA319900.026.37943653
TCGCCGT322750.025.8427552
CGTATCA322100.025.79710256
GTCGCCG337900.025.7406351
GCCGTAT342000.025.62658354
GGTCGCC342200.025.52959450
CGAGATC46950.025.201525
CTCGGTG312300.024.78983144
TGGTCGC344150.024.77463549
TCTCGGT320050.024.2659243
ATCTCGG324650.024.2019542
GTGGTCG360450.023.9940748
GTATCAT329700.023.94975557
GATCTCG317400.023.83955441
GGTGGTC373500.022.99608247
TCGGTGG372500.022.87912645
AGATCTC337700.022.71642340
TCATTAA372150.022.194860
CATTAAA382100.022.05610761
Produced by FastQC (version 0.11.5)